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MorphoMuseuM in press original article (anatomy atlas)

Original article : anatomy atlas

MicroCT survey of larval skeletal mineralization in the Cuban gar Atractosteus tristoechus (Actinopterygii; Lepisosteiformes)
Raphaël Scherrer, Andrés Hurtado, Erik Garcia Machado and Mélanie Debiais-Thibaud
Keywords: Actinopterygii; development; Lepisosteiformes; mineralization; skeleton

doi: 10.18563/m3.3.3.e3

Cite this article: Scherrer R., Hurtado A., Garcia Machado E., Debiais-Thibaud M., 2017. MicroCT survey of larval skeletal mineralization in the Cuban gar Atractosteus tristoechus (Actinopterygii; Lepisosteiformes). MorphoMuseuM 3 (3)-e3. doi: 10.18563/m3.3.3.e3

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Using X-ray microtomography, we describe the ossification events during the larval development of a non-teleost actinopterygian species: the Cuban gar Atractosteus tristoechus from the order Lepisosteiformes. We provide a detailed developmental series for each anatomical structure, covering a large sequence of mineralization events going from an early stage (13 days post-hatching, 21mm total length) to an almost fully ossified larval stage (118dph or 87mm in standard length). With this work, we expect to bring new developmental data to be used in further comparative studies with other lineages of bony vertebrates. We also hope that the on-line publication of these twelve successive 3D reconstructions, fully labelled and flagged, will be an educational tool for all students in comparative anatomy. 

Specimens and 3D Data

Atractosteus tristoechus At1-13dph View specimen


At1-13dph : 13 dph larvae, 21 mm TL

Type: "3D_surfaces"

3D view: doi: 10.18563/m3.sf.94

Data citation: Scherrer R., Hurtado A., Garcia Machado E., Debiais-Thibaud M., 2017. M3#94_At1-13dph. doi: 10.18563/m3.sf.94


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Atractosteus tristoechus At2-16dph View specimen


Atractosteus tristoechus larva, 16 dph, 26mm SL.

Type: "3D_surfaces"

3D view: doi: 10.18563/m3.sf.95

Data citation: Scherrer R., Hurtado A., Garcia Machado E., Debiais-Thibaud M., 2017. M3#95_At2-16dph. doi: 10.18563/m3.sf.95


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Atractosteus tristoechus At3-19dph View specimen


Atractosteus tristoechus larva, 19 dph, 27mm SL.

Type: "3D_surfaces"

3D view: doi: 10.18563/m3.sf.96

Data citation: Scherrer R., Hurtado A., Garcia Machado E., Debiais-Thibaud M., 2017. M3#96_At3-19dph. doi: 10.18563/m3.sf.96


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Atractosteus tristoechus At4-22dph View specimen


Atractosteus tristoechus larva, 22dph, 30mm SL.

Type: "3D_surfaces"

3D view: doi: 10.18563/m3.sf.97

Data citation: Scherrer R., Hurtado A., Garcia Machado E., Debiais-Thibaud M., 2017. M3#97_At4-22dph. doi: 10.18563/m3.sf.97


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Atractosteus tristoechus At5-26dph View specimen


Atractosteus tristoechus larva, 26 dph, 32mm SL.

Type: "3D_surfaces"

3D view: doi: 10.18563/m3.sf.98

Data citation: Scherrer R., Hurtado A., Garcia Machado E., Debiais-Thibaud M., 2017. M3#98_At5-26dph. doi: 10.18563/m3.sf.98


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Atractosteus tristoechus At6-31dph View specimen


Atractosteus tristoechus larva, 31 dph, 39mm SL.

Type: "3D_surfaces"

3D view: doi: 10.18563/m3.sf.99

Data citation: Scherrer R., Hurtado A., Garcia Machado E., Debiais-Thibaud M., 2017. M3#99_At6-31dph. doi: 10.18563/m3.sf.99


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Atractosteus tristoechus At7-37dph View specimen


Atractosteus tristoechus larva, 37 dph, 43mm SL.

Type: "3D_surfaces"

3D view: doi: 10.18563/m3.sf.100

Data citation: Scherrer R., Hurtado A., Garcia Machado E., Debiais-Thibaud M., 2017. M3#100_At7-37dph. doi: 10.18563/m3.sf.100


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Atractosteus tristoechus At8-52dph View specimen


Atractosteus tristoechus larva, 52 dph, 46mm SL.

Type: "3D_surfaces"

3D view: doi: 10.18563/m3.sf.101

Data citation: Scherrer R., Hurtado A., Garcia Machado E., Debiais-Thibaud M., 2017. M3#101_At8-52dph. doi: 10.18563/m3.sf.101


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Atractosteus tristoechus At9-74dph View specimen


Atractosteus tristoechus larva, 74 dph, 61mm SL. Not all structures are colored, only newly ossified ones.

Type: "3D_surfaces"

3D view: doi: 10.18563/m3.sf.102

Data citation: Scherrer R., Hurtado A., Garcia Machado E., Debiais-Thibaud M., 2017. M3#102_At9-74dph. doi: 10.18563/m3.sf.102


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Atractosteus tristoechus At10-89dph View specimen


Atractosteus tristoechus larva, 89 dph, 63mm SL. Not all structures are colored, only newly ossified ones. You may find the tag file in the At1-13dph reconstruction data.

Type: "3D_surfaces"

3D view: doi: 10.18563/m3.sf.103

Data citation: Scherrer R., Hurtado A., Garcia Machado E., Debiais-Thibaud M., 2017. M3#103_At10-89dph. doi: 10.18563/m3.sf.103


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Atractosteus tristoechus At11-104dph View specimen


Atractosteus tristoechus larva, 104 dph, 70mm SL. Not all structures are colored, only newly ossified ones.

Type: "3D_surfaces"

3D view: doi: 10.18563/m3.sf.104

Data citation: Scherrer R., Hurtado A., Garcia Machado E., Debiais-Thibaud M., 2017. M3#104_At11-104dph. doi: 10.18563/m3.sf.104


Download 3D data

Atractosteus tristoechus At12-118dph View specimen


Atractosteus tristoechus larva, 118 dph, 87mm SL.

Type: "3D_surfaces"

3D view: doi: 10.18563/m3.sf.105

Data citation: Scherrer R., Hurtado A., Garcia Machado E., Debiais-Thibaud M., 2017. M3#105_At12-118dph. doi: 10.18563/m3.sf.105


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in press


Arratia, G., 2009. Identifying patterns of diversity of the actinopterygian fulcra. Acta Zoologica. 90, 220–235.

Arratia, G., Schultze, H.-P., 1991. Palatoquadrate and Its Ossifications: Development and Homology Within Osteichthyans. Journal of Morphology. 208, 1–81.

Arratia, G., Schultze, H.-P., 1992. Reevaluation of the caudal skeleton of certain actinopterygian fishes: III. Salmonidae. Homologization of caudal skeletal structures. Journal of Morphology. 214, 187–249.

Betancur-R, R., Broughton, R.E., Wiley, E.O., Carpenter, K., López, J.A., Li, C., Holcroft, N.I., Arcila, D., Sanciangco, M., Ii, J.C.C., Zhang, F., Campbell, M.A., Ballesteros, J.A., Roa-varon, A., Willis, S., Borden, W.C., Hough, D.J., Lu, G., 2013. The Tree of Life and a New Classification of Bony Fishes. PLOS Currents Tree of Life. 732988, 1–45.

Boughner, J.C., Buchtová, M., Fu, K., Diewert, V., Hallgrímsson, B., Richman, J.M., 2007. Embryonic development of Python sebae - I: Staging criteria and macroscopic skeletal morphogenesis of the head and limbs. Zoology. 110, 212–230.

Britz, R., Johnson, G.D., 2010. Occipito-vertebral fusion in actinopterygians: conjecture, myth and reality. Part 1: Non-teleosts. In: Mesozoic Fishes 4-Homology and Phylogeny. J. S. Nelson, H.-P. Schultze, and M. V. H. Wilson (Eds.). Verlag Dr. Freidrich Pfeil, München. pp. 77–93.

Broughton, R.E., Betancur-R., R., Li, C., Arratia, G., Ortí, G., 2013. Multi-locus phylogenetic analysis reveals the pattern and tempo of bony fish evolution, PLoS Currents Tree of Life.

Cignoni, P., Cignoni, P., Callieri, M., Callieri, M., Corsini, M., Corsini, M., Dellepiane, M., Dellepiane, M., Ganovelli, F., Ganovelli, F., Ranzuglia, G., Ranzuglia, G., 2008. MeshLab: an Open-Source Mesh Processing Tool. Sixth Eurographics Italian Chapter Conference. 129–136.

Dean, M.N., Mull, C.G., Gorb, S.N., Summers, A.P., 2009. Ontogeny of the tessellated skeleton: Insight from the skeletal growth of the round stingray Urobatis halleri. Journal of Anatomy. 215, 227–239.

Dufeau, D.L., Witmer, L.M., 2015. Ontogeny of the Middle-Ear Air-Sinus System in Alligator mississippiensis (Archosauria: Crocodylia). PLoS ONE. 10, 1–25.

Enault S., Adnet S., Debiais-Thibaud M., 2016. Skeletogenesis during the late embryonic development of the catshark Scyliorhinus canicula (Chondrichthyes; Neoselachii). MorphoMuseuM 1 (4)-e2.

Friedman, M., 2015. The early evolution of ray-finned fishes. Palaeontology. 58, 213–228.

Gardiner, B.G., Littlewood, D.T.G., Maisey, J.G., 1996. Interrelationships of basal neopterygians. In: Stiassny, M.L.J., Parenti, L.R., Johnson, G.D. (Eds.), Interrelationships of Fishes. Academic Press, San Diego, California, pp. 117–146.

Gardiner, B.G., Schaeffer, B., 1989. Interrelationships of lower actinopterygian fishes. Zoological Journal of the Linnean Society. 1989, 135–187.

Gardiner, B.G., Schaeffer, B., Masserie, J.A., 2005. A review of the lower actinopterygian phylogeny. Zoological Journal of the Linnean Society. 144, 511–525.

Gegenbauer, C., 1887. Ueber die Occipitalregion und die ihr benachbarten Wirbel der Fische. In: Festschrift Albert von Kölliker Zur Feier Seines Siebenzigsten Geburtstages Gewidmet von Seinen Schülern. W. Engelmann, Leipzig.

Grande, L., 2010. An empirical synthetic pattern study of gars (Lepisosteiformes) and closely related species, based mostly on skeletal anatomy. The resurrection of Holostei., American Society of Ichthyologists and Herpetologists Special Publication 6:i–x, 1–871; supplementary issue of Copeia 10 (2A).

Grande, L., Bemis, W.E., 1991. Osteology and phylogenetic relationships of fossil and recent paddlefishes (Polyodontidae) with comments on the interrelationships of Acipenseriformes. Journal of Vertebrate Paleontology. 11, 1-121.

Grande, L., Bemis, W.E., 1998. A Comprehensive Phylogenetic Study of Amiid Fishes (Amiidae) Based on Comparative Skeletal Anatomy. an Empirical Search for Interconnected Patterns of Natural History. Journal of Vertebrate Paleontology. 18, 1–696.

Gregory, W.K.K., 1959. Fish skulls - A study of the evolution of natural mechanisms, Transactions of the American Philosophical Society. Eric Lundberg, Laurel, Florida.

Hammarberg, F., 1937. Zur Kenntnis der Ontogenetischen Entwicklung des Schädels von Lepisosteus platystomus. Acta Zoologica. 18, 210–336.

Helfman, G., Collette, B., Facey, D., 2009. The diversity of fishes—biology, evolution, and ecology, 2nd edition. Wiley-Blackwell, Oxford, UK.

Hilton, E.J., Konstantinidis, P., Schnell, N.K., Dillman, C.B., 2014. Identity of a unique cartilage in the buccal cavity of gars (Neopterygii: Lepisosteiformes: Lepisosteidae). Copeia. 2014, 50–55.

Hilton, E.J., Schnell, N.K., Konstantinidis, P., 2015. When Tradition Meets Technology: Systematic Morphology of Fishes in the Early 21st Century. Copeia. 103, 858–873.

Ho, T.V., Iwata, J., Ho, H.A., Grimes, W.C., Park, S., Sanchez-Lara, P.A., Chai, Y., 2015. Integration of comprehensive 3D microCT and signaling analysis reveals differential regulatory mechanisms of craniofacial bone development. Developmental Biology. 400, 180–190.

Hurley, I.A., Mueller, R.L., Dunn, K.A., Schmidt, E.J., Friedman, M., Ho, R.K., Prince, V.E., Yang, Z., Thomas, M.G., Coates, M.I., 2007. A new time-scale for ray-finned fish evolution. Proceedings. Biological sciences / The Royal Society. 274, 489–498.

Huysseune, A., Sire, J.-Y., 1998. Evolution of patterns and processes in teeth and tooth-related tissues in non-mammalian vertebrates. European journal of oral sciences. 106, 437–481.

Jessen, H., 1972. Schultergürtel und Pectoralflosse bei Actinopterygiern. Fossil Strata. 1, 1–101.
Jollie, M., 1984. Development of Cranial and Pectoral Girdle Bones of Lepisosteus with a Note on Scales. Copeia. 2, 476–502.

Kammerer, C.F.F., Grande, L., Westneat, M.W.W., 2006. Comparative and Developmental Functional Morphology of the Jaws of Living and Fossil Gars (Actinopterygii : Lepisosteidae). Journal of morphology. 267, 1017–31.

Konstantinidis, P., Warth, P., Naumann, B., Metscher, B., Hilton, E.J., Olsson, L., 2015. The Developmental Pattern of the Musculature Associated with the Mandibular and Hyoid Arches in the Longnose Gar, Lepisosteus osseus (Actinopterygii, Ginglymodi, Lepisosteiformes). Copeia. 103, 920–932.

Kryzanovsky, S., 1927. Die Entwicklung der paarigen Flossen bei Acipenser, Amia und Lepidosteus. Acta Zoologica. 8, 278–352.

Lauder, G. V., 1980. Evolution of the feeding mechanism in primitive actionopterygian fishes: A functional anatomical analysis of Polypterus, Lepisosteus, and Amia. Journal of Morphology. 163, 283–317.

Lebrun, R., 2014. ISE-MeshTools, a 3D interactive fossil reconstruction freeware. In: 12th Annual Meeting of EAVP, Torino, Italy.

Lecointre, G., Philippe, H., Van Le, H.L., Le Guyader, H., 1994. How many nucleotides are required to resolve a phylogenetic problem? The use of a new statistical method applicable to available sequences. Molecular Phylogenetics and Evolution.

Long, J.M., Snow, R.A., 2016. Ontogenetic development of otoliths in alligator gar. Transactions of the American Fisheries Society. 145, 537–544.

Long, W.L., Ballard, W.W., 2001. Normal embryonic stages of the longnose gar, Lepisosteus osseus. BMC developmental biology. 1, 6.

López-Arbarello, A., 2012. Phylogenetic Interrelationships of Gynglymodian Fishes (Actinopterygii: Neopterygii). PLoS ONE. 7, 1–44.

Luther, A., 1913. Ueber die vom N. trigeminus versorgte Muskulatur der Ganoiden und Dipneusten. Helsingfors Acta Societas Scientiarum Fennica. 41, 1–72.

Maxwell, E.E., Wilson, L.A.B., 2013. Regionalization of the axial skeleton in the “ambush predator” guild -- are there developmental rules underlying body shape evolution in ray-finned fishes? BMC Evolutionary Biology. 13, 265.

Mayhew, R.L., 1924. The skull of Lepidosteus platostomus. Journal of Morphology. 38, 315–346.

Metscher, B.D., 2009. MicroCT for developmental biology: A versatile tool for high-contrast 3D imaging at histological resolutions. Developmental Dynamics. 238, 632–640.

Near, T.J., Eytan, R.I., Dornburg, A., Kuhn, K.L., Moore, J.A., Davis, M.P., Wainwright, P.C., Friedman, M., Smith, W.L., 2012. Resolution of ray-finned fish phylogeny and timing of diversification. Proceedings of the National Academy of Sciences. 109, 13698–13703.

Nelson, J.S., Grande, T.C., M. V. H. W, 2016. Order LEPISOSTEIFORMES - gars. In: Fishes of the World. John Wiley & Sons, New York.

Normark, B.B., McCune,  a R., Harrison, R.G., 1991. Phylogenetic relationships of neopterygian fishes, inferred from mitochondrial DNA sequences. Molecular biology and evolution. 8, 819–834.

Palci, A., Lee, M.S.Y., Hutchinson, M.N., 2016. Patterns of postnatal ontogeny of the skull and lower jaw of snakes as revealed by micro-CT scan data and three-dimensional geometric morphometrics. Journal of Anatomy.

Perry, S.F., Wilson, R.J. a, Straus, C., Harris, M.B., Remmers, J.E., 2001. Which came first, the lung or the breath? Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology. 129, 37–47.

Rice, R., Kallonen, A., Cebra-Thomas, J., Gilbert, S.F., 2016. Development of the turtle plastron, the order-defining skeletal structure. Proceedings of the National Academy of Sciences of the United States of America. 113, 5317–5322.

Rücklin, M., Donoghue, P.C.J., Cunningham, J.A., Marone, F., Stampanoni, M., 2014. Developmental Paleobiology of the Vertebrate Skeleton. Journal of Paleontology. 88, 676–683.

Schindelin, J., Arganda-Carreras, I., Frise, E., Kaynig, V., Longair, M., Pietzsch, T., Preibisch, S., Rueden, C., Saalfeld, S., Schmid, B., Tinevez, J.-Y., White, D.J., Hartenstein, V., Eliceiri, K., Tomancak, P., Cardona, A., 2012. Fiji: an open-source platform for biological-image analysis. Nature Methods. 9, 676–682.

Schmalhausen, J.J., 1913. Zur Morphologie der unpaaren Flossen. II. Bau und Phylogenese der unpaaren Flossen und insbesonders der Schwanzflosse der Fische. Zeitschrift für wissenschaftliche Zoologie. 104, 1–80.

Schreiner, K.E., 1902. Einige Ergebnisse über den Bau und der Occipitalregion von Amia und Lepidosteus. Zetischriften. wiss. Zool. 72, 467–524.

Schultze, H.-P., Cloutier, R., 1991. Computed Tomography and Magnetic Resonance Imaging studies of Latimeria chalumnae. Environmental Biology of Fishes. 32, 159–181.

Sewertzoff, A.N., 1895. Die Entwicklung der Occipitalregion der niederen Vertebraten im Zusammenhang mit der Frage nach der Metamerie des Kopfes. Bulletin de la société impériale des naturalistes de Moscou. 9, 186–284.

Shen, L., Ai, H., Liang, Y., Ren, X., Anthony, C.B., Goodlett, C.R., Ward, R., Zhou, F.C., 2013. Effect of prenatal alcohol exposure on bony craniofacial development: a mouse MicroCT study. Alcohol (Fayetteville, N.Y.). 47, 405–15.

Sire, J.-Y., 1989. Scales in Young Polypterus senegalus Are Elasmoid : New Phylogenetic ImpIications. The American Journal of Anatomy. 186, 315–323.

Sire, J.-Y., 1990. From ganoid to elasmoid scales in the actinopterygian fishes. Netherlands journal of zoology. 40, 75–92.

Sire, J.-Y., Donoghue, P.C.J., Vickaryous, M.K., 2009. Origin and evolution of the integumentary skeleton in non-tetrapod vertebrates. Journal of Anatomy. 214, 409–440.

Sire, J.-Y., Géraudie, J., Meunier, F.J., Zylberberg, L., 1987. On the Origin of Ganoine: Histological and Ultrastructural Data on the Experimental Regeneration of the Scales of Calamoichthys calabaricus (Osteichthyes, Brachyopterygii, Polypteridae). The American Journal of Anatomy. 180, 391–402.

Slater, B.J., Liu, K.J., Kwan, M.D., Quarto, N., Longaker, M.T., 2009. Cranial osteogenesis and suture morphology in Xenopus laevis: A unique model system for studying craniofacial development. PLoS ONE. 4.

Veit, O., 1907. Über einige Besonderheiten am Primordialcranium von Lepidosteus osseus. Anatomische Hefte. 99, 155–204.

Vickaryous, M.K., Sire, J.Y., 2009. The integumentary skeleton of tetrapods: Origin, evolution, and development. Journal of Anatomy. 214, 441–464.

Wiley, E.O., 1976. The phylogeny and biogeography of fossil and recent gars (Actinopterygii: Lepisosteidae). University of Kansas Museum of Natural History Miscellaneous Publications. 64, 1–111.