Considerable morphological variations are found in the middle ear among mammals. Here I present a three-dimensional atlas of the middle ear ossicles of eulipotyphlan mammals. This group has radiated into various environments as terrestrial, aquatic, and subterranean habitats independently in multiple lineages. Therefore, eulipotyphlans are an ideal group to explore the form-function relationship of the middle ear ossicles. This comparative atlas of hedgehogs, true shrews, water shrews, mole shrews, true moles, and shrew moles encourages future studies of the middle ear morphology of this diverse group.